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qiagen buffer pe  (Qiagen)


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    Structured Review

    Qiagen qiagen buffer pe
    Qiagen Buffer Pe, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+buffer/Buffer+PE/pm42127909-725-19-19
    Average 97 stars, based on 578 article reviews
    qiagen buffer pe - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: Striking convergent selection history of wheat and barley and its potential for breeding.
    Article Snippet: Two washing steps were performed by adding 750 μl PE buffer (Qiagen) to the MinElute column, spinning at 3,300g for 1 min and discarding the flowthrough.

    Article Title: Experimental Evolution of Yeast Reveals Trade-offs Between Early and Late Stationary Phase
    Article Snippet: The pellet was then washed twice with 500 μL of PE buffer and resuspended in PE buffer (Qiagen) during the last wash.

    Article Title: Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination.
    Article Snippet: Carefully open the glass plates and stain the gel in 100 mL of deionized water with 10 μL of SYBR Gold (Invitrogen) for 10 min. 35.

    Article Title: Striking convergent selection history of wheat and barley and its potential for breeding
    Article Snippet: Two washing steps were performed by adding 750 μl PE buffer (Qiagen) to the MinElute column, spinning at 3,300 g for 1 min and discarding the flowthrough.

    Article Title: Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination
    Article Snippet: Add 750 μL of PE buffer (Qiagen) and centrifuge at 13,000 rpm (15,871 x g) for 1 min. 48.


    Article Title: Probing the zooarchaeological record across time and space for ancient pathogen DNA
    Article Snippet: Samples were then filtered through minElute columns and washed twice with 200 μL PE buffer (Qiagen) before elution in 50 μL EBT.

    Modification:

    Article Title: Microbial community dynamics in a traditional Swiss mountain cheese over 142 years of cheesemaking
    Article Snippet: .. The mixture was then transferred to a high pure extender assembly (Roche Applied Science, UV treated) modified with a MinElute column (QIAGEN), centrifuged until all the sample solution was passed through, and washed twice with 700 μl PE buffer (QIAGEN) before elution in 100 μl of TET (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20, pH 8.0). ..



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    Image Search Results


    BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Expressing, Western Blot, Staining, Control

    The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Biomarker Discovery, Flow Cytometry, Endpoint Dilution Assay, Expressing, Immunofluorescence, Staining, Knockdown, Fluorescence